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 | Acceso al texto completo restringido a Biblioteca INIA La Estanzuela. Por información adicional contacte bib_le@inia.org.uy. |
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha : |
07/06/2019 |
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Actualizado : |
07/06/2019 |
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Tipo de producción científica : |
Poster |
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Autor : |
GARMENDIA, G.; PATTARINO. L.; NEGRIN, C.; MARTÍNEZ-SILVEIRA, A.; PEREYRA, S.; VERO, S.; WARD, T.J |
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Afiliación : |
GABRIELA GARMENDIA, Universidad de la República, Facultad de Química, Montevideo, Uruguay.; LUCIA PATTARINO, Universidad de la República, Facultad de Química, Montevideo, Uruguay.; CAMILA NEGRIN TORRES, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ADALGISA MARTÍNEZ SILVEIRA, Universidad de la República, Facultad de Química, Montevideo, Uruguay.; SILVIA ANTONIA PEREYRA CORREA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; SILVANA VERO, Universidad de la República, Facultad de Química, Montevideo, Uruguay.; TODD J. WARD, United States Department of Agriculture, Agricultural Research Service, Peoria, IL, USA. |
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Título : |
Species composition, toxigenic potential and aggressiveness of Fusarium isolates causing Head Blight of barley in Uruguay.[Poster]. |
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Fecha de publicación : |
2018 |
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Fuente / Imprenta : |
In: Proceedings of the Fusarium head blight Forum, December 2-4, 2018, Regency St. Louis at the Arch St. Louis, Missouri, USA. |
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Páginas : |
p. 84. |
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Idioma : |
Inglés |
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Contenido : |
ABSTRACT:Fusarium Head Blight (FHB) represents one of the major constraints for barley production in Uruguay and significantly decreases grain yield and quality. FHB is also a major food safety concern because causal agents contaminate grains with trichothecenes and other mycotoxins. DNA sequence-based analyses and in-vitro toxin assessments were used to characterize species and trichothecene chemotype composition of FHB pathogens on barley grains in Uruguay. Fusarium graminearum was the dominant species (89.7%), and three other members of the F. graminearum species complex (FGSC) were identified
as FHB pathogens of barley in Uruguay for the first time. Other minor FHB species included F. poae,F. avenaceum, F. pseudograminearum and an unnamed species of the F. incarnatum-equiseti species complex (FIESC). Most isolates (89.7%) had the 15-acetyldeoxynivalenol (15-ADON) trichothecene
type. Yet, results from this study expanded the known area of occurrence within Uruguay for the nivalenol (NIV) toxin type, which was observed among isolates from three species of the FGSC, F. pseudograminearum, and F. poae. Isolates with the 3-acetyldeoxynivalenol (3-ADON) or NX-2 toxin types were not observed, although a previously published multilocus genotyping assay was updated to identify NX-2 strains. Analyses of population structure and comparisons with FHB isolates from wheat in Uruguay indicated that F. graminearum constitutes a single genetic population with no evidence of population differentiation related to the sampled hosts. Inter and intraspecific differences were observed in aggressiveness toward four barley genotypes with different levels of resistance to FHB, and in general nivalenol producers were the least aggressive isolates. Sensitivity to metconazole was approximately
10 times higher than the one detected for tebuconazole. This is the first report regarding tebuconazole
and metconazole sensitivity for Fusarium species causing FHB in barley in Uruguay and establishes
an important starting point for monitoring temporal or spatial changes in FGSC sensitivity, which is
critical to define FHB management practices. MenosABSTRACT:Fusarium Head Blight (FHB) represents one of the major constraints for barley production in Uruguay and significantly decreases grain yield and quality. FHB is also a major food safety concern because causal agents contaminate grains with trichothecenes and other mycotoxins. DNA sequence-based analyses and in-vitro toxin assessments were used to characterize species and trichothecene chemotype composition of FHB pathogens on barley grains in Uruguay. Fusarium graminearum was the dominant species (89.7%), and three other members of the F. graminearum species complex (FGSC) were identified
as FHB pathogens of barley in Uruguay for the first time. Other minor FHB species included F. poae,F. avenaceum, F. pseudograminearum and an unnamed species of the F. incarnatum-equiseti species complex (FIESC). Most isolates (89.7%) had the 15-acetyldeoxynivalenol (15-ADON) trichothecene
type. Yet, results from this study expanded the known area of occurrence within Uruguay for the nivalenol (NIV) toxin type, which was observed among isolates from three species of the FGSC, F. pseudograminearum, and F. poae. Isolates with the 3-acetyldeoxynivalenol (3-ADON) or NX-2 toxin types were not observed, although a previously published multilocus genotyping assay was updated to identify NX-2 strains. Analyses of population structure and comparisons with FHB isolates from wheat in Uruguay indicated that F. graminearum constitutes a single genetic population with no evidence of population dif... Presentar Todo |
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Palabras claves : |
BARLEY; FUSARIUM HEAD BLIGHT(FHB); TIZÓN DE LA CABEZA. |
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Thesagro : |
CEBADA; MICOTOXINAS. |
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Asunto categoría : |
H20 Enfermedades de las plantas |
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Marc : |
LEADER 02985nam a2200253 a 4500
001 1059832
005 2019-06-07
008 2018 bl uuuu u00u1 u #d
100 1 $aGARMENDIA, G.
245 $aSpecies composition, toxigenic potential and aggressiveness of Fusarium isolates causing Head Blight of barley in Uruguay.[Poster].$h[electronic resource]
260 $aIn: Proceedings of the Fusarium head blight Forum, December 2-4, 2018, Regency St. Louis at the Arch St. Louis, Missouri, USA.$c2018
300 $ap. 84.
520 $aABSTRACT:Fusarium Head Blight (FHB) represents one of the major constraints for barley production in Uruguay and significantly decreases grain yield and quality. FHB is also a major food safety concern because causal agents contaminate grains with trichothecenes and other mycotoxins. DNA sequence-based analyses and in-vitro toxin assessments were used to characterize species and trichothecene chemotype composition of FHB pathogens on barley grains in Uruguay. Fusarium graminearum was the dominant species (89.7%), and three other members of the F. graminearum species complex (FGSC) were identified as FHB pathogens of barley in Uruguay for the first time. Other minor FHB species included F. poae,F. avenaceum, F. pseudograminearum and an unnamed species of the F. incarnatum-equiseti species complex (FIESC). Most isolates (89.7%) had the 15-acetyldeoxynivalenol (15-ADON) trichothecene type. Yet, results from this study expanded the known area of occurrence within Uruguay for the nivalenol (NIV) toxin type, which was observed among isolates from three species of the FGSC, F. pseudograminearum, and F. poae. Isolates with the 3-acetyldeoxynivalenol (3-ADON) or NX-2 toxin types were not observed, although a previously published multilocus genotyping assay was updated to identify NX-2 strains. Analyses of population structure and comparisons with FHB isolates from wheat in Uruguay indicated that F. graminearum constitutes a single genetic population with no evidence of population differentiation related to the sampled hosts. Inter and intraspecific differences were observed in aggressiveness toward four barley genotypes with different levels of resistance to FHB, and in general nivalenol producers were the least aggressive isolates. Sensitivity to metconazole was approximately 10 times higher than the one detected for tebuconazole. This is the first report regarding tebuconazole and metconazole sensitivity for Fusarium species causing FHB in barley in Uruguay and establishes an important starting point for monitoring temporal or spatial changes in FGSC sensitivity, which is critical to define FHB management practices.
650 $aCEBADA
650 $aMICOTOXINAS
653 $aBARLEY
653 $aFUSARIUM HEAD BLIGHT(FHB)
653 $aTIZÓN DE LA CABEZA
700 1 $aPATTARINO. L.
700 1 $aNEGRIN, C.
700 1 $aMARTÍNEZ-SILVEIRA, A.
700 1 $aPEREYRA, S.
700 1 $aVERO, S.
700 1 $aWARD, T.J
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Registro original : |
INIA La Estanzuela (LE) |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha actual : |
21/02/2014 |
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Actualizado : |
01/10/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
B - 2 |
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Autor : |
COZZOLINO, D.; DELUCCHI, M.I.; KHOLI, M.; VÁZQUEZ, D. |
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Afiliación : |
DANIEL COZZOLINO GÓMEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARIA INES DELUCCHI ZAPARRART, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MOHAM KHOLI, MOHAM, International Center for Wheat and Maize Improvement (CIMMYT).; DANIEL VÁZQUEZ PEYRONEL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Use of near infrared reflectance spectroscopy to evaluate quality characteristics in whole-wheat grain. [Uso de la espectroscopía de reflectancia en el infrarrojo cercano para evaluar características de calidad en trigo]. |
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Fecha de publicación : |
2006 |
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Fuente / Imprenta : |
Agricultura Técnica, December 2006, Volume 66, Issue 4, Pages 370-375. |
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DOI : |
10.4067/S0365-28072006000400005 |
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Idioma : |
Inglés |
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Notas : |
Article history:Recibido: 17 de octubre de 2005/Aprobado: 30 de marzo de 2006. |
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Contenido : |
ABSTRACT:
The aim of this work was to explore the potential of visible (Vis) and near infrared reflectance (NIR) spectroscopy to measure quality characteristics in whole grain wheat (Triticum aestivum L.) as a tool in breeding programs. A total of 100 samples were analyzed by the reference methods for crude protein (CP), wet gluten (WG) and sodium dodecyl sulfate (SDS) sedimentation test. Whole grain samples were scanned in a NIR monochromator instrument (400-2500 nm) in reflectance. Partial least squares (PLS) were used to develop calibration equations for the quality characteristics in whole wheat. Calibration models were validated using an independent set of samples (n = 50) randomly selected from the population set. The uncertainty of the PLS models was evaluated by the standard error of prediction (SEP). The SEP obtained were 0.35% for CP, 2.04 for SDS and 4.14% for WG. It was concluded that NIR spectroscopy might be used as a screening tool to segregate early generations of wheat genotypes. At a later stage is needed to improve the accuracy of the NIR calibrations, broadening the calibration spectra with the incorporation of more genotypes and different crop years.
RESUMEN:
El objetivo de este trabajo fue explorar el potencial de la espectroscopía en el visible (Vis) e infrarrojo cercano (NIR) para medir características de calidad en el trigo (Triticum aestivum L.) para su uso en programas de mejoramiento. Cien muestras fueron analizadas por el método de referencia para proteína cruda (CP), gluten húmedo (WG) y sulfato de dodecil de sodio (SDS) o prueba de sedimentación. Las muestras de trigo se analizaron en un instrumento NIR (400-2500 nm) en reflectancia. El método de los cuadrados mínimos parciales (PLS) fue utilizado para desarrollar las ecuaciones de calibración para las características de calidad en trigo. Los modelos de calibración se validaron utilizando un conjunto independiente de muestras (n = 50) aleatoriamente escogido del conjunto de la población. La incertidumbre de los modelos PLS de calibración fue evaluada usando el error estándar de la predicción (SEP). El SEP obtenido fue de 0,35% para CP, 2,04 para SDS y 4,14% para WG. Se concluyó que la espectroscopía de NIR podría utilizarse como una herramienta de selección para segregar genotipos de trigo en generaciones tempranas. En una etapa posterior se necesita mejorar la precisión de los análisis NIR, ampliando el espectro de calibración con la incorporación de más genotipos y diferentes años de cultivo. MenosABSTRACT:
The aim of this work was to explore the potential of visible (Vis) and near infrared reflectance (NIR) spectroscopy to measure quality characteristics in whole grain wheat (Triticum aestivum L.) as a tool in breeding programs. A total of 100 samples were analyzed by the reference methods for crude protein (CP), wet gluten (WG) and sodium dodecyl sulfate (SDS) sedimentation test. Whole grain samples were scanned in a NIR monochromator instrument (400-2500 nm) in reflectance. Partial least squares (PLS) were used to develop calibration equations for the quality characteristics in whole wheat. Calibration models were validated using an independent set of samples (n = 50) randomly selected from the population set. The uncertainty of the PLS models was evaluated by the standard error of prediction (SEP). The SEP obtained were 0.35% for CP, 2.04 for SDS and 4.14% for WG. It was concluded that NIR spectroscopy might be used as a screening tool to segregate early generations of wheat genotypes. At a later stage is needed to improve the accuracy of the NIR calibrations, broadening the calibration spectra with the incorporation of more genotypes and different crop years.
RESUMEN:
El objetivo de este trabajo fue explorar el potencial de la espectroscopía en el visible (Vis) e infrarrojo cercano (NIR) para medir características de calidad en el trigo (Triticum aestivum L.) para su uso en programas de mejoramiento. Cien muestras fueron analizadas por el método de refer... Presentar Todo |
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Palabras claves : |
GLUTEN HÚMEDO; GRAIN QUALITY; GRANO DE TRIGO; PROTEIN; SDS; WET GLUTEN; WHOLE WHEAT. |
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Thesagro : |
NIRS; PROTEÍNA; TRIGO; TRITICUM AESTIVUM. |
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Asunto categoría : |
F01 Cultivo |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/13383/1/Uso-de-la-espectroscopia-de-reflectancia-en-el-inf.pdf
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Marc : |
LEADER 03624naa a2200313 a 4500
001 1034984
005 2019-10-01
008 2006 bl uuuu u00u1 u #d
024 7 $a10.4067/S0365-28072006000400005$2DOI
100 1 $aCOZZOLINO, D.
245 $aUse of near infrared reflectance spectroscopy to evaluate quality characteristics in whole-wheat grain. [Uso de la espectroscopía de reflectancia en el infrarrojo cercano para evaluar características de calidad en trigo].$h[electronic resource]
260 $c2006
500 $aArticle history:Recibido: 17 de octubre de 2005/Aprobado: 30 de marzo de 2006.
520 $aABSTRACT: The aim of this work was to explore the potential of visible (Vis) and near infrared reflectance (NIR) spectroscopy to measure quality characteristics in whole grain wheat (Triticum aestivum L.) as a tool in breeding programs. A total of 100 samples were analyzed by the reference methods for crude protein (CP), wet gluten (WG) and sodium dodecyl sulfate (SDS) sedimentation test. Whole grain samples were scanned in a NIR monochromator instrument (400-2500 nm) in reflectance. Partial least squares (PLS) were used to develop calibration equations for the quality characteristics in whole wheat. Calibration models were validated using an independent set of samples (n = 50) randomly selected from the population set. The uncertainty of the PLS models was evaluated by the standard error of prediction (SEP). The SEP obtained were 0.35% for CP, 2.04 for SDS and 4.14% for WG. It was concluded that NIR spectroscopy might be used as a screening tool to segregate early generations of wheat genotypes. At a later stage is needed to improve the accuracy of the NIR calibrations, broadening the calibration spectra with the incorporation of more genotypes and different crop years. RESUMEN: El objetivo de este trabajo fue explorar el potencial de la espectroscopía en el visible (Vis) e infrarrojo cercano (NIR) para medir características de calidad en el trigo (Triticum aestivum L.) para su uso en programas de mejoramiento. Cien muestras fueron analizadas por el método de referencia para proteína cruda (CP), gluten húmedo (WG) y sulfato de dodecil de sodio (SDS) o prueba de sedimentación. Las muestras de trigo se analizaron en un instrumento NIR (400-2500 nm) en reflectancia. El método de los cuadrados mínimos parciales (PLS) fue utilizado para desarrollar las ecuaciones de calibración para las características de calidad en trigo. Los modelos de calibración se validaron utilizando un conjunto independiente de muestras (n = 50) aleatoriamente escogido del conjunto de la población. La incertidumbre de los modelos PLS de calibración fue evaluada usando el error estándar de la predicción (SEP). El SEP obtenido fue de 0,35% para CP, 2,04 para SDS y 4,14% para WG. Se concluyó que la espectroscopía de NIR podría utilizarse como una herramienta de selección para segregar genotipos de trigo en generaciones tempranas. En una etapa posterior se necesita mejorar la precisión de los análisis NIR, ampliando el espectro de calibración con la incorporación de más genotipos y diferentes años de cultivo.
650 $aNIRS
650 $aPROTEÍNA
650 $aTRIGO
650 $aTRITICUM AESTIVUM
653 $aGLUTEN HÚMEDO
653 $aGRAIN QUALITY
653 $aGRANO DE TRIGO
653 $aPROTEIN
653 $aSDS
653 $aWET GLUTEN
653 $aWHOLE WHEAT
700 1 $aDELUCCHI, M.I.
700 1 $aKHOLI, M.
700 1 $aVÁZQUEZ, D.
773 $tAgricultura Técnica, December 2006, Volume 66, Issue 4, Pages 370-375.
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